Application
Sp1 transcription factor has been used as a standard in electrophoretic mobility shift assay.
Biochem/physiol Actions
Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned and characterized. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a well-characterized motif found in several other transcription factors. In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
| biological source | human |
| recombinant | expressed in insect cells |
| assay | ≥75% (SDS-PAGE) |
| form | frozen liquid |
| mol wt | ~81.6 kDa |
| packaging | pkg of 5 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | 200 μg/mL |
| technique(s) | electrophoretic mobility shift assay: suitable |
| color | clear colorless |
| NCBI accession no. | NM_138473 |
| UniProt accession no. | P08047 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... SP1(6667) |
