General description
Probable ATP-dependent RNA helicase DDX5 (UniProt: P17844; also known as EC:3.6.4.13, DEAD box protein 5, DDX5, RNA helicase p68) is encoded by the DDX5 (also known as G17P1, HELR, HLR1) gene (Gene ID: 1655) in human. DDX5 is a member of the DEAD box protein family that is characterized by a conserved Asp-Glu-Ala-Asp (DEAD; aa 248-251) motif within a helicase core. It contains a helicase ATP-binding domain (aa 125-300) and a helicase C-terminal domain (aa 328-475). It is present in cytoplasm and in the nucleus. During the G0 phase, it is predominantly located in the nucleus. Its cytoplasmic levels increase during the G1/S phase and during the M phase it is located in the vicinity of the condensed chromosomes. DDX5 is an ATP-dependent RNA helicase that participates in unwinding of RNA secondary structure, RNA splicing, mRNA export, transcript stability, rRNA biogenesis, and microRNA processing. It is also involved in transcriptional regulation and this function is independent of its RNA helicase activity. It also serves as a transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. DDX5 is essential for regulation of appropriate splicing of key genes necessary for spermatogenesis. It regulates expression of cell cycle genes in undifferentiated spermatogonia post-transcriptionally and is required for cell proliferation and survival. Overexpression of DDX5 has been reported in several human cancers and is implicated in tumorigenesis. It activates the Myc expression in multiple solid tumors. It is reported to preferentially unfold the Myc GQ structure to activate Myc expression in several cancer cell lines. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wu, G., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(41); 20453-20461; Legrand, JMD., et al. (2019). Nat. Commun. 10(1); Article number: 2278; Bates GJ., et al. (2005). EMBO J. 24(3); 543-553).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Probable ATP-dependent RNA helicase DDX5.
Application
Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DDX5 in SH-SY5Y cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DDX5 in C6, NIH3T3, Cos7, and U2OS cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DDX5 in human cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected DDX5 in SH-SY5Y cells.
Affinity Binding Assay: A representative lot of this antibody bound DDX5 with a KD of 6.3 x 10-9 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 4K14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects DDX5. It targets an epitope within 19 amino acids from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4K14, recombinant monoclonal |
| description | 4K14 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 69.15 kDa,observed mol wt ~60 kDa |
| purified by | using Protein A |
| species reactivity | rat, human, mouse, monkey |
| species reactivity (predicted by homology) | canine, porcine, bovine, feline |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_001307524 |
| UniProt accession no. | P17844 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... DDX5(1655) |
