General description
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.Alpaca derived anti-PCSK9 VHH single domain antibody (molecular weight: 14.4 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Immunogen
Recombinant human PCSK9 (Gln31-Gln692) protein
Application
ELISA, IHC, IF
Biochem/physiol Actions
Human Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9)
Packaging
50 µg per vial / 100 µg per vial
Physical form
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.12
Preparation Note
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Stable for 1 year at 4°C from date of receipt.
Other Notes
Storage at 4°C; Do not freeze.
| biological source | alpaca |
| Quality Segment | 100 |
| recombinant | expressed in E. coli |
| conjugate | unconjugated |
| antibody form | affinity purified VHH antibody |
| clone | LDL7, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | lyophilized solid |
| mol wt | 14.4 kDa, ~15-18 kDa (SDS PAGE under reducing condition) |
| purified by | immobilized metal affinity chromatography (IMAC) |
| species reactivity | human |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:3000-1:10000,immunofluorescence: 1:200-1:600,immunohistochemistry: 1:200-1:600 |
| color | colorless |
| pH | ( in PBS, pH 7.4) |
| isotype | VHH |
| Protein ID accession no. | Q8NBP7.3 |
| UniProt accession no. | Q8NBP7 |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PCSK9(255738) |
