General description
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
Immunogen
Recombinant human HLA-E denaturated heavy chain.
Biochem/physiol Actions
The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.
Physical form
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified by protein-A affinity chromatography.
Store at 2-8°C. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | unconjugated |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | MEM-E/02, monoclonal |
| form | buffered aqueous solution |
| species reactivity | ( Non-human primates), human |
| concentration | 1 mg/ml |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P13747 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... HLA-E(3133) |
