Biochem/physiol Actions
MNK2 (MAP kinase-interacting kinase 2) contains a conserved C-terminal ERK-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain. MNK2 binds tightly to the growth factor-regulated MAP kinases, ERK1 and ERK2. ERK and p38 phosphorylate MNK2, which stimulates its in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E).A yeast two-hybrid screen showed the MNK2 protein interacted with the ligand-binding domain of estrogen receptor beta (ERbeta).
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
| recombinant | expressed in baculovirus infected Sf9 cells |
| Quality Segment | 200 |
| product line | PRECISIO® Kinase |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | 81-109 nmol/min·mg |
| mol wt | ~74 kDa |
| UniProt accession no. | Q9HBH9 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... MKNK2(2872) |
