Immunogen
recombinant human interferon-γ, expressed and purified from Escherichia coli.
Biochem/physiol Actions
Interferons (IFNs) are cytokines that are secreted in response to viral or bacterial infections and tumorigenesis. IFN-γ binds to specific receptor complex consisting of IFNγR1 and IFNγR2. There are many pathways that are mediated by IFN-γ binding such as JAK/STAT1, AP-1, NF-κB, STAT3 and STAT5. IFN-γ is pleotropic and performs various functions related to immune response, inflammation, differentiation and activation of T cells, cell cycle and apoptosis.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.
Preparation Note
Purified using protein A affinity chromatography.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 25718, monoclonal |
| form | lyophilized powder |
| species reactivity | human |
| technique(s) | immunocytochemistry: 8-25 μg/mL using immersion fixed human peripheral blood mononuclear cells stimulated with PMA and ionomycin,neutralization: suitable |
| impurities | endotoxin, tested |
| isotype | IgG2a |
| UniProt accession no. | P01579 |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... IFNG(3458) |
