General description
IgM antibody is produced by B cells and has pentamer structure which helps the antibody in polyreactivity and can also remove apoptotic cells. Anti-human IgM (μ-chain specific) -alkaline phosphatase antibody can be used in solid phase ELISA for determination of anti-sp75 antibodies. Goat anti human IgM-alkaline phosphatase antibody reacts specifically with human IgM.
Immunogen
Human IgM.
Application
IgM是一种糖蛋白，重链上具有5个n连接的糖基化位点。可通过ELISA分析确定结合凝集素的IgM的糖基化形式，其中碱性磷酸酶标记的山羊抗人IgM可作为二抗（1:2000稀释），以对硝基苯基为底物(Sigma)。
IgM是一种糖蛋白，重链上有5个n连接糖基化位点。可通过ELISA分析确定结合凝集素的IgM的糖基化形式，其中碱性磷酸酶标记的山羊抗人IgM可作为二抗（1:2000稀释），以对硝基苯基为底物(Sigma)。Arnold, J. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin.JBC, 280: 29080-29087.
It may be used for immunoblotting.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Segment | 200 |
| conjugate | alkaline phosphatase conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| species reactivity | human |
| technique(s) | direct ELISA: 1:7,000-1:21,000 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
