General description
Research area: Cell Signaling
IGFBPs (insulin-like growth factor-binding proteins) are mainly required to regulate IGFs (insulin like growth factors) activities. IGFBP3 is a multifunctional protein and is secreted by various cells. It is most ample circulating IGFBP and works in IGF-dependent and -independent mechanisms. The IGFBP3 gene is mapped to human chromosome 7p12.3.
Recombinant human IGF-BP3 is a 28.8kDa protein consisting of 264 amino acid residues.
Application
IGF-Binding Protein-3 human has been used to study its association with follicular fluid and subsequent effect on embryo development.
Biochem/physiol Actions
IGFBP3 (insulin-like growth factor-binding protein 3) is involved in cell survival as well as cell death and tumorigenesis. Epigenetic changes in IGFBP3 affect non-small-cell lung cancer, hepatocellular carcinoma, ovarian cancer, skin cancer, urological cancers, breast cancer and gastric cancer. IGFBP3 is a primary growth inhibitor and can reverse the mitogenic functions of IGF-1 (insulin like growth factor 1) by inducing apoptosis.IGFBP-3 functions both in an IGF-dependent and independent manner. Its IGF-dependent roles involve endocrine function by delivering IGFs to target cells and activating downstream signaling. Meanwhile, its IGF-independent roles include interactions with extracellular matrix and plasma membrane proteins, as well as translocation into the cytoplasm and nucleus. The C-terminal domain has cell-penetrating abilities, enabling the generation of short peptides for intracellular delivery of drugs or genes.
Physical form
Lyophilized from 20mM HCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| potency | ≤0.2 μg/mL |
| mol wt | 28.8 kDa |
| packaging | pkg of 25 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white to off-white |
| suitability | suitable for molecular biology |
| UniProt accession no. | P17936 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... IGFBP3(3486) |
