Application
Useful for Kinase assay and Western blot.
Biochem/physiol Actions
Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system (1). PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding (2). PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | 70-95% (SDS-PAGE) |
| form | frozen liquid |
| specific activity | 2.0-3.5 nmol/min·mg (radiometric) |
| mol wt | ~64 kDa (Cyclin Y), ~72 kDa (PFTK1 (CDK14)) |
| concentration | 0.1 μg/μL |
| accession no. | NM_012395,NM_145012 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... CCNY(219771), CDK14(5218) |
