General description
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.
Immunogen
A mixture of U266 and XG-1 human myeloma cell lines
Biochem/physiol Actions
The mouse monoclonal antibody MI15 recognizes an extracellular epitope of CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | FITC conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | MI15, monoclonal |
| form | buffered aqueous solution |
| species reactivity | ( Human), rat, ( Non-human primates) |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| UniProt accession no. | P18827 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SDC1(6382) |
