General description
Interferon-induced GTP-binding protein Mx1 (UniProt: P20591; also known as Interferon-induced protein p78, IFI-78K, Interferon-regulated resistance GTP-binding protein MxA, Myxoma resistance protein 1, Myxovirus resistance protein 1) is encoded by the MX1 gene (Gene ID: 4599) in human. MX1/MxA is a homotetrameric, interferon-induced dynamin-like GTPase that can oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain (aa 367-632). This oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures. Its C-terminal GTPase effector domain (GED; aa 574-662) is involved in oligomerization and viral target recognition. Its middle domain (aa 366-533) mediates self-assembly and oligomerization. It displays antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. It is reported to antagonize reoviridae and asfarviridae replication and inhibit thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. It differs from other small GTPases and heterotrimeric G proteins in features such as their large size (70-100 kDa), a relatively low affinity for GTP, and a high intrinsic rate of GTP hydrolysis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Verhelst, J., et al (2013). Microbiol. Mol. Biol. Rev. 77(4); 551-566; Bridgen, A., et al. (2004). Virus Res. 99(1); 47-50; Kochs, G., et al. (2002). Proc. Natl. Acad. Sci. USA. 99(5):3153-3158).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human MX1/MxA.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MX1/MxA in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected MX1/MxA in lysates from A549 cells and 1:1,000 dilution in Human thymus tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MX1/MxA in Human thymus tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MX1/MxA in A549 cells.
Affinity Binding Assay: A representative lot of this antibody bound MX1/MxA peptide with a KD of 1.2 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2C20 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects MX1/MxA. It targets an epitope within 21 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2C20, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 75.52 kDa,observed mol wt ~76 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_002453.2 |
| UniProt accession no. | P20591 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... MX1(4599) |
