General description
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Immunogen
Human IgG-kappa myeloma protein
Biochem/physiol Actions
The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | FITC conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | TB28-2, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
