Anti-phospho-CD3ζ (pTyr⁷²) antibody produced in rabbit，100UL，SAB4200330-100UL，Sigma

General description

T cell antigen receptor ζ chain (CD3ζ), also known as CD247 gene, spanning 88kb of genomic DNA, is mapped to human chromosome 1q24.2.
The T cell antigen receptor-CD3 (TCR-CD3) multimeric complex is composed of TCRαβ, CD3γε, CD3δε and ζ ζ dimers. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs.

Immunogen

synthetic phosphopeptide corresponding to residues surrounding Tyr⁷² of mouse CD3ζ, conjugated to KLH. The corresponding sequence differs by a single amino acid in human.

Application

Anti-phospho-CD3ζ (pTyr72) antibody has been used in

• immunoblotting
• immunoprecipitation
• western blotting

Biochem/physiol Actions

T cell antigen receptor ζchain (CD3ζ) functions as a key signal transduction component of the T cell antigen receptor (TCR) complex. The encoded protein facilitates optimal effector T-cell function by enhancing receptor expression and signaling. Mutation in the gene increases the risk of susceptibility to systemic lupus erythematosus (SLE). Reduced expression of the gene has been observed in T-cells of cancer, lupus and chronic infectious diseases such as leprosy and tuberculosis patients. CD3ζ serves as a potent biomarker for determining progression and severity in patients with type 2 diabetes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.