General description
Mammals contain variants of histone H3, that are, the replicative histones (H3.1 and H3.2) and the replacement histones (H3.3). Histone H3 is located on human chromosome 6p22.
Immunogen
The epitope recognized by PLA0148 maps to a region between residue 100 and the C-terminus (residue 135) of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350).
Biochem/physiol Actions
Histone proteins are the basic building blocks of chromatin. Histone H3 controls protein-protein interactions to induce binding of trans-acting factors that have a role in chromatin condensation. Histone H3 K27M mutations are observed in adult cerebellar high-grade gliomas.
Physical form
Tris-buffered Saline containing 0.1% BSA with 0.09% Sodium Azide
Other Notes
Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| species reactivity | human |
| technique(s) | immunohistochemistry: 1:200-1:1,000,immunoprecipitation (IP): 2-10 μg/mg,western blot: 1:2,000- 1:10,000 |
| accession no. | NP_003520.1 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | rabbit ... Histone H3(8350) |
