General description
DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.
DARPP32 phosphorylated on Thr75 acts as an inhibitor of PKA.
Immunogen
synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.
Application
Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.
Biochem/physiol Actions
Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac®.
Physical form
Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
Other Notes
The labeling by the antibody to DARPP32 Thr75 is markedly increased by okadaic acid treatment.
Legal Information
Prozac is a registered trademark of Eli Lilly and Co.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| usage | sufficient for 10 blots |
| mol wt | antigen 32 kDa |
| species reactivity | mouse, human |
| technique(s) | dot blot: 1:1,000,western blot: 1:1,000 |
| UniProt accession no. | Q9UD71 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr75) |
| Gene Information | human ... PPP1R1B(84152) mouse ... Ppp1r1b(19049) |
