产品介绍
Product Description
General description
FLAG BioM2小鼠单克隆抗体通过酰肼键与生物素共价连接。该抗体可识别FLAG融合蛋白N末端、Met-N末端或C末端的FLAG序列。
Application
生物素标记抗体被用于使用亲和素或链霉亲和素偶联报告酶(如链霉亲和素-过氧化物酶)的免疫检测方法。 当使用小鼠细胞作为重组蛋白宿主时,首选一抗偶联物。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。
请访问我们的 FLAG®应用 门户网站,了解更多产品详情。
Physical form
溶于50%甘油,10 mM磷酸钠,pH 7.25,含有150mM NaCl和0.02%叠氮化钠
Preparation Note
用TBS(0.05M Tris,pH7.4,含有0.15M NaCl)稀释抗体至终浓度为1-10μg/mL。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
技术参数
Specifications
| biological source | mouse |
| Quality Level | 200 |
| conjugate | biotin conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | M2, monoclonal |
| form | buffered aqueous glycerol solution |
| species reactivity | all |
| concentration | ~1 mg/mL |
| technique(s) | dot blot: suitable (chemiluminescent detection) |
| isotype | IgG1 |
| immunogen sequence | DYKDDDDK |
| shipped in | dry ice |
| storage temp. | −20°C |

