General description
The Lenti CRISPR Non-Target gRNA Control Transduction Particles (LV02 vector) include a gRNA sequence that does not target known human, mouse and rat genes. It is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-target) and Ef1a-driven Puromycin resistance cassette. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Application
Functional Genomics/Screening/Target Validation
Other Notes
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.
Legal Information
CRISPR Use License Agreement
Lentiviral, WPRE, and Evrogen Licenses
| form | liquid |
| Quality Segment | 200 |
| packaging | vial of 8 x 25 μL |
| concentration | (5x108 TU/ml (via p24 titering assay)) |
| application(s) | CRISPR |
| shipped in | dry ice |
| storage temp. | −70°C |
