General description
Insulin-like growth factor 2 (UniProt: P01344; also known as Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, Insulin-like growth factor II Ala-25 Del, Preptin) is encoded by the IGF2 (also known as IGF-2; ORF: PP1446) gene (Gene ID: 3481) in human. IGF2 is a key growth factor, composed of a single polypeptide chain stabilized by disulfide bonds, sharing significant homology with insulin. IGF2 plays a pivotal role in fetal development, cell proliferation, and differentiation by binding to IGF receptors, including IGF1R and IGF2R, and insulin receptors. Its activity is modulated by IGF-binding proteins (IGFBPs), which regulate its bioavailability and receptor interactions. IGF2 is predominantly expressed during embryogenesis, with lower levels persisting in adult tissues such as the liver and brain. IGF2 resides in the extracellular matrix and circulates in plasma, where it interacts with IGFBPs to mediate endocrine and paracrine signaling. Dysregulation of IGF2 expression, often through epigenetic alterations, has been implicated in various diseases, including cancer, Beckwith-Wiedemann syndrome, and Silver-Russell syndrome, where aberrant IGF2 signaling contributes to tumorigenesis and growth abnormalities. IGF2 also participates in metabolic pathways, influencing glucose homeostasis and lipid metabolism. Its involvement in disease highlights its therapeutic potential, with strategies targeting IGF2 signaling pathways under investigation for cancer and metabolic disorders. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal half of human IGF2.
Application
Quality Control Testing
Evaluated by Western Blotting in HepG2 conditioned media.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IGF2 in HepG2 conditioned media.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected a recombinant fragment of human IGF2.
Affinity Binding Assay: A representative lot of this antibody bound with IGF2 peptide with at least one billion-fold (1,000,000,000X) higher affinity than with non-specific control peptide.
Enzyme Immunoassay: A representative lot of this antibody detected IGF2 protein in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2G15 is a ZooMAb® rabbit recombinant monoclonal antibody that detects IGF2. It targets an epitope within 22 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Other Notes
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2G15, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 20.14 kDa,observed mol wt ~10-20 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_000603 |
| UniProt accession no. | P01344 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... IGF2(3481) |
