General description
Monoclonal Anti-ARG1 (mouse IgG1 isotype) is derived from the hybridoma ARG1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Arginase (ARG)1 gene, codes for type I isoform of arginase. ARG1 is a cytosolic enzyme expressed predominantly in the liver as a component of the urea cycle.
Immunogen
synthetic peptide corresponding to a sequence at the N-terminal region of human ARG1, conjugated to KLH. The corresponding sequence differs by 3 amino acids in mouse ARG1 and by 4 amino acids in rat ARG1.
Application
Monoclonal Anti-ARG1 antibody produced in mouse has been used in immunoblotting.
Biochem/physiol Actions
Arginase hydrolyzes arginine to ornithine and urea. Arginases have been implicated in many disease processes, including vascular diseases, pulmonary diseases, infectious diseases, immune cell dysfunction and cancer. Inherited deficiency of ARG1 enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | ARG1-3, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~40 kDa |
| species reactivity | rat, human, mouse |
| concentration | ~1.0 mg/mL |
| technique(s) | western blot: 2-4 μg/mL using whole extracts of rat or mouse liver. |
| UniProt accession no. | P05089 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ARG1(383) |
