General description
Cluster of differentiation 20 (CD20), also referred to as membrane spanning 4-domains A1 (MS4A1), is expressed on the surface of human B lymphocytes and on a small subset of T cells. The 33-35kDa, non-glycosylated protein has four membrane-spanning domains with both the amino and carboxy termini of the protein localized to the cytoplasm and a short 43 residue-extracellular segment. It is part of the membrane-spanning 4A gene family. The gene encoding MS4A1 is localized on human chromosome 11q12-13.
Immunogen
Raji human Burkitt′s lymphoma cell line
Application
The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 10 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Biochem/physiol Actions
Cluster of differentiation 20 (CD20) participates in the development and cell cycle progression in B-cells. It has been found to interact with proteins like adapter protein p75/80, CD40 and major histocompatibility complex class II proteins (MHC II). It may participate in Ca2+ influx across plasma membranes, maintenance of Ca2+ concentration and activation of B-cells. CD20 activates Src leading to rapid phosphorylation of phospholipase C-γ1 (PLC-γ1) and PLC-γ2, in turn activating caspase-3 signaling of apoptosis. Overexpression of the gene has been associated with the development of primary cutaneous T-cell lymphomas (CTCL).
Features and Benefits
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Physical form
Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MEM-97, monoclonal |
| form | buffered aqueous solution |
| species reactivity | pig, bovine, human |
| concentration | 1 mg/mL |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_021950.3 |
| UniProt accession no. | P11836 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... MS4A1(931) |
