General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Reticulon-3 (UniProt O95197; also known as HAP, Homolog of ASY protein, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II, NSP-like protein 2, NSP-like protein II, NSPLII) is encoded by the RTN3 (also known as ASYIP, NSPL2) gene (Gene ID 10313) in human. Reticulon-3/RTN3 belongs to the RTN family of proteins characterized by their C-terminal reticulon homolog domain (RHD; a.a. 844-1032 in RTN3). There exist four mammalian reticulons (RTN1-4), with each member having multiple spliced isoforms. Human RTN3 is a multipass ER and golgi membrane protein with 3 membrane helical regions (a.a. 864-997, 948-968, 973-993) and four cytoplasmic regions (a.a. 2-863, 888-947, 969-972, 994-1032). Its C-terminal RHD mediates interaction with FADD (a.a. 987-1032) and BACE1 (a.a.1000-1002). RTN3 is expressed in neurons and localized in axons and growth cones. Seven isoforms of RTN3 have been described that are produced by alternative splicing. Isoform 3 is widely expressed, with highest levels in brain and three times more abundant in macula than in peripheral retina. Isoform 1, is expressed at high levels in brain and at low levels in skeletal muscle whereas Isoform 2 is only found in melanoma. RTN3 overexpression in neurons results in reduced amyloid deposition in murine models of Alzheimer′s disease (AD). Consistently, RTN3 deficiency elevates BACE1 protein level and enhances amyloid deposition in AD mice that express human mutant APP and presenilin-1 transgenes. RTN3 may act as a candidate protein in regulating the pathogenesis of atherosclerosis (AS) and Alzheimer s disease. It may also be involved in axonal transport (increase of this protein causes its imbalance) and its accumulation in swollen neuritis. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: He, W., et al. (2004). Nat. Med.10(9):959-965); He, W., et al. (2007). J. Biol. Chem. 282(40):29144-29151).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal cytoplasmic domain of human RTN3.
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from human brain tissue.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RTN3 in human brain tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected RTN3 in lysate from U-87 MG cells.
Affinity Binding Assay: A representative lot of this antibody bound RTN3 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RTN3 in U-2 OS cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected RTN3 in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2G21-H1 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects RTN3. It targets an epitope within 17 amino acids from the N-terminal cytoplasmic domain. This antibody can detect mutiple Isoforms of RTN3.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2G21-H1, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 23.48 kDa,observed mol wt ~23/120 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | chimpanzee |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal cytoplasmic domain |
| Protein ID accession no. | NP_001252518 |
| UniProt accession no. | O95197 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... RTN3(10313) |
