General description
Cullin 3 (CUL3) is a member of cullin protein family and is encoded by the gene mapped to human chromosome 2q36.2. The encoded protein is a core component of cullin/RING E3 ubiquitin ligase (CRL) and is ubiquitously expressed. The protein is characterized with a cullin homology domain.
Monoclonal Anti-Cullin 3 (mouse IgG1 isotype) is derived from the hybridoma CUL3-9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Cullin 3.
Application
Monoclonal Anti-Cullin 3 antibody produced in mouse has been used in western blotting and immunoprecipitation.
Biochem/physiol Actions
Cullin 3 (CUL3) acts as a scaffold protein and facilitates the organization of a large number of ubiquitin ligase complexes. It plays a vital role in the regulation of electrolyte homeostasis and blood pressure. The encoded protein acts as a key factor in protein ubiquitination and is also implicated in early embryonic development. One important role of CUL3 is to promote invasion and migration of trophoblast cells. Mutation in the gene leads to onset of pre-eclampsia and pseudohypoaldosteronism type II.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | CUL3-9, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen 80 kDa |
| species reactivity | rat, human, mouse |
| concentration | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable,western blot: 0.5-1.0 μg/mL using whole extracts of human Jurkat cells or mouse brain. |
| isotype | IgG1 |
| UniProt accession no. | Q13618 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CUL3(8452) mouse ... Cul3(26554) rat ... Cul3(301555) |
