Biochem/physiol Actions
BCA-1 is a potent chemoattractant for B lymphocytes, but not T lymphocytes, monocytes, or neutrophils.
BCA-1 is a potent chemoattractant for B lymphocytes, but not T lymphocytes, monocytes, or neutrophils. Its specific receptor BLR1 is a G protein-coupled receptor originally isolated from Burkitt′s lymphoma cells. Among cells of the hematopoietic lineages, the expression of BRL1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells.
Potent chemoattractant for B lymphocytes, but not T lymphocytes, monocytes, or neutrophils.
The biological activity of human BLC/BCA-1 is measured by its ability to chemoattract mouse BaF/3 cells transfected with human CXCR5.
Physical form
Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA containing 50 μg bovine serum albumin per 1 μg of cytokine.
| biological source | human |
| Quality Segment | 200 |
| recombinant | expressed in E. coli |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.1000 μg/mL |
| mol wt | apparent mol wt 8.7 kDa by SDS-PAGE (reducing) |
| packaging | pkg of 25 μg |
| storage condition | avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.) |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | O43927 |
| storage temp. | −20°C |
| Gene Information | human ... CXCL13(10563) |
