General description
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Homer protein homolog 1 (UniProt: Q86YM7; also known as Homer-1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID: 9456) in human. Homer protein homolog 1 (Homer-1) is a scaffolding protein involved in intracellular signaling pathways and neuronal function. It plays a critical role in receptor clustering, cell signaling modulation, and synaptic plasticity. Homer-1 contains an N-terminal coiled-coil region that allows for the formation of homo- and heteromeric interactions with other proteins, while the C-terminal domain binds to various target proteins involved in synaptic signaling. Homer-1 interacts with several proteins such as metabotropic glutamate receptors (mGluRs), inositol trisphosphate receptors (IP3Rs), and Shank proteins. Homer-1 is widely expressed in various tissues, but it is highly enriched in the brain, particularly in regions associated with synaptic transmission, such as the cortex, hippocampus, and cerebellum. Dysregulation of Homer-1 expression and function has been implicated in various neurological disorders, including schizophrenia, Alzheimer′s disease, epilepsy, and drug addiction. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Luo, P., et al., (2012) Neurochem. Int. 61, 731 738)
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of Homer-1.
Application
Quality Control Testing
Evaluated by Western Blotting in Neuro-2a cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Homer-1 in Neuro-2a cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in lysates from SH-SY5Y cells and Human brain tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in Rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Homer-1 in SH-SY5Y cells.
Affinity Binding Assay: A representative lot of this antibody bound Homer-1 peptide with a KD of 1.0 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1F5 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Homer-1. It targets an epitope within 17 amino acids from the C-terminal region of human Homer-1.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1F5, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 40.28 kDa,observed mol wt ~51 kDa |
| purified by | using Protein A |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | bovine, monkey, avian, canine, (Whale), horse |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_004263.1 |
| UniProt accession no. | Q86YM7 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... HOMER1(9456) |
