General description
OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.
Application
Functional Genomics/Target Validation
- Epigenetic Modification
- Transcriptional Activation
Features and Benefits
Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.
Other Notes
1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).
Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.
To order U6-gRNA plasmids, please visit sigma.com/crisprs
Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.
Legal Information
CRISPR Use License Agreement
Lentiviral and WPRE License Agreements
| Quality Segment | 200 |
| form | liquid |
| packaging | vial of 50 μL |
| concentration | 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| application(s) | CRISPR |
| shipped in | dry ice |
| storage temp. | −20°C |
