General description
The melanocyte-specific protein PMEL (also known as Pmel17, gp100, melanocyte protein, premelanosome protein or silver locus protein homolog (SILV) is a type I transmembrane glycoprotein that is expressed primarily in pigment cells of the eye and skin. In melanosomes, PMEL protein forms a fibrillar matrix on which the UV-shielding pigment melanin is deposited.1-2
Application
The antibody may be used in various immunochemical techniques including Immunoblotting (~100 kDa), Immunohistochemistry and Immunofluorescence.
Biochem/physiol Actions
Monoclonal Anti-PMEL antibody specifically recognizes human PMEL protein.
PMEL fibrils are a major functional component of the melanosomal compartment as they optimize the melanin polymerization, condensation and storage.1,3
In addition, PMEL fibrils have an amyloidogenic nature sharing features with pathological amyloids.4 Thus, PMEL is suggested as an excellent model system to study mechanisms of intracellular amyloid formation.1
Mutations in PMEL are associated with pigmentation disorders and/or impairments in eye development in various species.1,5,6
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| conjugate | unconjugated |
| Quality Segment | 200 |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | PL-19 |
| form | liquid |
| species reactivity | human |
| concentration | ~1 mg/mL |
| technique(s) | immunoblotting: 1-2 μg/mL using human melanoma SK-MEL-28 cell lysate,immunofluorescence: 1-2 μg/mL using human melanoma SK-MEL-28 cells |
| isotype | IgG1 |
| UniProt accession no. | P40967 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PMEL(6490) |
