General description
IκBα is an inhibitor of the NFκB complex and inactivates the NFκB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IκB protein by IκB kinases (IKKs) marks it for destruction via the ubiquitination pathway, thereby allowing the activation of the NFκB complex. Synthetic glucocorticoid such as dexamethasone display anti-inflammatory effects by inducing the increased synthesis of the IκB protein thereby inhibiting the activity of the NFκB complex. Overexpression of the IκBα gene in fibroblasts leads to inhibition of production of IL-6, TNF receptor, MMP-1, MMP-3 and MMP-13.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
| recombinant | expressed in E. coli |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~61 kDa |
| NCBI accession no. | NM_020529 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... NFKBIA(4792) |
