General description
Period circadian protein homolog 2 (UniProt: O15055; also known as hPER2, Circadian clock protein PERIOD 2, PER2) is encoded by the PER2 (also known as KIAA0347) gene (Gene ID: 8864) in human. PER2 is a homodimeric protein that is widely expressed and is high levels are found in skeletal muscle and pancreas. It serves as a transcriptional repressor and is a core component of the circadian clock along with other clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) it plays a critical role in rhythm generation. Its levels oscillate diurnally, and its rhythmic levels are critical for the generation of circadian rhythms in central as well as peripheral clocks. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK/NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2 heterodimer inhibiting its activity. PER2 shuttles between the nucleus and cytoplasm and this shuttling is affected by interaction with other circadian core oscillator proteins and/or by phosphorylation. It translocates to the nucleus after phosphorylation by CSNK1D or CSNK1E. PER2 contains a nuclear localization signal (aa 789-805) and three nuclear export signals (aa 111-120, 462-471, and 989-996). Mutations in PER2 gene have been linked to advanced sleep phase syndrome. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Period circadian protein homolog 2 (PER2).
Application
Anti-PER2, clone 1G17 ZooMAb, Cat. No. ZRB1076, is a recombinant Rabbit monoclonal antibody that specifically targets PER2 and has been tested for use in Affinity Binding Assay, Immunocytochemistry, and Immunohistochemistry (Paraffin).
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PER2 in human retina tissue sections.
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected PER2 in HeLa cells.
Affinity Binding Assay: A representative lot of this antibody bound PER2 peptide with a KD of 7.2 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1G17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Period circadian protein homolog 2 (PER2). It targets an epitope within 21 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1G17, recombinant monoclonal,monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 136.58 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| isotype | IgG |
| UniProt accession no. | O15055 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PER2(8864) |
