General description
PRDX6 (peroxiredoxin 6) is a mammalian peroxidase belonging to the 1-Cys Prdx family, meaning it has 1-Cys at position 47 (Cys47). This protein is localized to cytoplasm, lysosomes, mitochondria, endoplasmic reticulum (ER) and plasma membrane. It is a homodimeric protein which is especially observed in mammalian lungs.†
Application
Anti-Peroxiredoxin 6 (C-terminal) antibody produced in rabbit has been used for Western Blotting.
Biochem/physiol Actions
PRDX6 (peroxiredoxin 6) has both peroxidase and phospholipase A2 (PLA2) activities and thus, is a bifunctional protein. In liver, as a peroxidase it confers protection against damage from ROS (reactive oxygen species) during ischemia-reperfusion injury. As a PLA2 it is involved in lung surfactant metabolism. It is also essential for normal nicotinamide adenine dinucleotide phosphate-oxidase activity. In the livers of donors after brain death (DBD), this protein suppresses ischemia- and hypoxia-induced liver damage, and its expression is controlled by NF-κB (nuclear factor). In human and mouse neuronal cells, the delivery of PRDX6 protein leads to delay or suppression in oxidative stress-induced neuronal cell death.
Peroxiredoxin 6 contains a single redox-active cysteine and uses glutathione to catalyze the reduction of H2O2 and other organic peroxides. Overexpression of peroxiredoxin 6 in cells protects them against oxidative damage, whereas knockdown of this enzyme results in oxidative stress and apoptosis. The phospholipase A2 activity plays an important role in surfactant homeostasis. Peroxiredoxin 6 is a major antioxidant enzyme which functions in antioxidant defense and lung phospholipid metabolism.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~25 kDa |
| species reactivity | human, rat, mouse |
| concentration | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): 2-5 μg using whole extract of mouse brain,western blot: 0.5-1.0 μg/mL using whole extract of human HeLa cells.,western blot: 1-2 μg/mL using whole extract of rat brain |
| UniProt accession no. | P30041 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PRDX6(9588) mouse ... Prdx6(11758) rat ... Prdx6(94167) |
