General description
DC-SIGN Monoclonal Antibody: Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR are known to exisit. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Immunogen
A recombinant His-tagged protein fragment corresponding to the extracellular region of human DC-SIGN.
Application
DC-SIGN antibody can be used for detection of DC-SIGN in Western Blot at 1 - 2 mg/mL and in immunohistochemistry at 5 - 10 mg/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Physical form
Supplied in PBS containing 0.02% sodium azide.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin (IgG) |
| antibody product type | primary antibodies |
| clone | monoclonal |
| form | liquid |
| species reactivity | human |
| concentration | 1 mg/mL |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1, kappa |
| UniProt accession no. | Q9NNX6 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CD209(30835) |
