General description
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Sheep anti-mouse IgG (whole molecule) F(ab′)2 fragment is specific for the F(ab′)2 of mouse IgG. The antibody preparation consists only of the F(ab′)2 fragment of sheep IgG as determined by SDS-PAGE. No contamination with sheep IgG whole molecule is observed.
Immunogen
Mouse IgG
Application
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000), western blot (1:30,000), and immunohistochemistry (at 1:50 dilution in formalin-fixed, paraffin-embedded sections). The antibody may also be used for dot blot and immunoblot assays using a working dilution of 1:30,000.
ELISAs using alkaline phosphatase conjugated sheep anti-mouse IgG whole molecule antibody were performed to measure the antibody response in the sera of mice immunixed to T. crassiceps. 1:1000 at 37 degrees for one hour and developed using p-nitrophenyl (Sigma).
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | sheep |
| Quality Segment | 200 |
| conjugate | alkaline phosphatase conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| species reactivity | mouse |
| technique(s) | direct ELISA: 1:30,000,dot blot: 1:30,000,immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50,western blot: 1:30,000 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
