Application
适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和
Biochem/physiol Actions
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Physical form
含 Tris 缓冲盐的冻干粉
Other Notes
One unit will oxidize 1.0 μmole of
| type | Type V |
| Quality Segment | 200 |
| form | lyophilized powder |
| specific activity | 250-750 units/mg protein |
| purified by | chromatography |
| foreign activity | lactate dehydrogenase ≤0.05%,malate dehydrogenase ≤0.1% |
| storage temp. | −20°C |
