Application
The Crystallography Cryo Kit for Proteins provides a rapid, empirical, and efficient screening method to determine the solubility and optimal starting conditions for the crystallization of biological macromolecules in the presence of a cryoprotectant (primarily glycerol). The screening range and probing ability of the Cryo Kit are comparable to those of the Basic Kit. A cryoprotectant is added to freeze crystals in an amorphous glass at -173°C.
Biochem/physiol Actions
Based on known or published data from various proteins, the Cryo Kit uses a sparse matrix method and minimal amount of protein sample to explore a broad range of buffers, pH, and precipitants. The Protein Cryo Kit is based on the "salting out" principal: increasing the concentration of a precipitating reagent and cryoprotectant depletes the macromolecules of bound water and leads to their precipitation or crystallization.
Features and Benefits
- Comprehensive, efficient screening
- Stringent purity standards
- Prefabricated reagents, flow charts, and observation sheets
- Low incidence of undesirable solubility effects
Other Notes
The kit contains 50 components with 10 mL each. Most components can be ordered separately as 100 mL packages.
| storage temp. | 2-8°C |
