Biochem/physiol Actions
Recombinant human RAS1 (2-186, G12V) was expressed in E.coli cells using an N-terminal His tag. The HRAS1(G12V) mutant is constitutively active. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer.
Preparation Note
Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Other Notes
2-186
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | 70-95% (SDS-PAGE) |
| form | frozen liquid |
| mol wt | ~84 kDa |
| concentration | 0.1 μg/μL |
| accession no. | NM_003159 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... HRAS1(6792) |
