General description
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells. Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa. GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain. GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells. While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling.Alpaca derived anti-GFAP VHH single domain antibody (molecular weight: 13.2 kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Immunogen
Recombinant human GFAP (Met1-Met432) protein
Application
WB, IHC,IF
Biochem/physiol Actions
Human Glial Fibrillary Acidic Protein (GFAP)
Packaging
50 µg per vial / 100 µg per vial
Physical form
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.10
Preparation Note
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Stable for 1 year at 4°C from date of receipt.
Other Notes
Storage at 4°C; Do not freeze.
| biological source | alpaca |
| Quality Segment | 100 |
| recombinant | expressed in E. coli |
| conjugate | unconjugated |
| antibody form | affinity purified VHH antibody |
| clone | GA11, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | lyophilized solid |
| mol wt | 13.2 kDa, ~15-18 kDa (SDS PAGE under reducing condition) |
| purified by | immobilized metal affinity chromatography (IMAC) |
| species reactivity | mouse, human |
| concentration | 1 mg/mL |
| technique(s) | immunofluorescence: 1:100-1:500,immunohistochemistry: 1:200-1:600,western blot: 1:1000-1:5000 |
| color | colorless |
| pH | ( in PBS, pH 7.4) |
| isotype | VHH |
| Protein ID accession no. | AAB22581.1 |
| UniProt accession no. | P14136 |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... GFAP(2670) |
