General description
Monoclonal Anti-Pro LC3C antibody (mouse IgG1 isotype) is derived from the BTY-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse. Human microtubule-associated proteins 1 light chain 3 (LC3C) is an ortholog of yeast Atg8. It is expressed in different human tissues. LC3C belongs to the subfamily of LC3 and the family of human Atg8. It is a structural protein of the autophagosomal membrane. LC3C gene is located on the human chromosome at 1q43.
Immunogen
Synthetic peptide corresponding to the C-terminal region of human LC3C, conjugated to KLH
Application
Anti-Pro LC3C antibody, mouse monoclonal may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Human microtubule-associated proteins 1 light chain 3 (LC3C) protein is considered as an autophagy marker. It enhances the trafficking of hepatocyte growth factor (Met) receptor tyrosine kinase (RTK) to an autophagic compartment. LC3C protein also plays a role in antibacterial autophagy.
Monoclonal Anti-Pro LC3C antibody specifically recognizes epitope at the C-terminal pro-peptide domain of human LC3C-I and/or LC3C-II and dose not cross react with human LC3A or LC3B.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody product type | primary antibodies |
| clone | BTY-1, monoclonal |
| form | liquid |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| concentration | ~1 mg/mL |
| technique(s) | immunoblotting: 0.5-1 μg/mL using whole extracts of HEK-293T cells overexpressing human LC3C |
| isotype | IgG1 |
| UniProt accession no. | Q9BXW4 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... MAP1LC3C(440738) |
