产品介绍
Product Description
General description
The roles of the residues in the catalytic active site of pyrimidine nucleoside phosphorylase from Bacillis subtilis have been elucidated using hybrid quantum-mechanical/molecular-mechanical methods.
Biochem/physiol Actions
Pyrimidine nucleoside phosphorylase functions in the nucleotide synthesis salvage pathway by catalyzing the reversible phosphorolysis of pyrimidines. Both uridine and thymidine are substrates.
Other Notes
One unit will convert 1 μmole each of 2′-deoxyuridine and phosphate to uracil and 2-deoxyribose 1-phosphate per minute at pH 7.4 and 37 °C.
技术参数
Specifications
| recombinant | expressed in E. coli |
| Quality Segment | 200 |
| form | lyophilized powder |
| specific activity | ≥70 units/mg protein |
| storage temp. | −20°C |
