General description
Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.
Immunogen
purified human IgE myeloma protein
Application
Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.
Biochem/physiol Actions
Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
| biological source | mouse |
| Quality Level | 200 |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | GE-1, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | ~1.0 mg/mL |
| technique(s) | ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating,immunoblotting: suitable,immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma |
| isotype | IgG2b |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
