产品介绍
Product Description
Application
Useful for citrullination and Western blot.
Biochem/physiol Actions
The five full length recombinant PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. This mixture is ideally suited for in vitro citrullination of protein targets.
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
技术参数
Specifications
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | 70-95% (SDS-PAGE) |
| form | frozen liquid |
| mol wt | ~95-100 kDa |
| concentration | 0.5 μg/μL (0.1 ug/uL each PAD) |
| accession no. | BC025718,BC049947,NM_013358,NM_016233,NM_207421 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PADI1(29943), PADI2(11240), PADI3(51702), PADI4(23569), PADI6(353238) |
