General description
Trans-Golgi network integral membrane protein 2 (TGOLN2), also known as trans-Golgi network 46 (TGN46), is encoded by the gene mapped to human chromosome 2p11.2. It is a heterodimeric type I integral membrane protein. TGOLN2 has a highly conserved N terminus, which consists of a signal peptide. The C terminus possesses a lumenal domain, a membrane spanning region and cytoplasmic tail.
Immunogen
synthetic peptide corresponding to the C-terminal region of human TGN46, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 3 amino acids in rat and mouse TGN46.
Biochem/physiol Actions
Trans-Golgi network integral membrane protein 2 (TGOLN2) cycles between the trans-Golgi network (TGN) and the cell surface via an early endosomal compartment. This movement is mediated by a tyrosine-based tetra peptide signal (SDYQRL) in the cytoplasmic domain. It plays an important role in the formation of exocytic vesicles at the TGN by functioning as a receptor for complexes of a cytoplasmic protein known as p62 and one GTP-binding protein. The cytoplasmic domain of TGOLN2 binds to the complex and is essential for budding process. It mediates the coupling of the segregation of secretory proteins to the budding of exocytic vesicles. The cytosolic domain of TGOLN2 interacts with AP2 clathrin adaptor complexes and also with the coiled coil region of a protein called neurabin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | TGN46-8, monoclonal |
| form | buffered aqueous solution |
| mol wt | 80-100 kDa |
| species reactivity | human |
| concentration | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using HeLa cells,western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing human TGN46 |
| isotype | IgG1 |
| UniProt accession no. | O43493 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TGOLN2(10618) |
