Immunogen
synthetic peptide corresponding to residues 122-136 of human CNPase. The sequence is 93% identical in mouse and rat.
Application
Anti-CNPase antibody produced in rabbit is suitable for flow cytometry at a working dilution of 1:200-1:500, immunohistochemistry at 1:100-1:200 and western blotting at 1:500-1:1000. It was used at a working dilution of 1:1000 as a primary antibody in western blotting of cerebrospinal fluid samples in a study.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Biochem/physiol Actions
CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase) is associated with brain tubulin and acts as a microtubule-associated protein in promoting microtubule assembly and distribution. It is expressed primarily in committed oligodendroglial progenitors and terminally-differentiated oligodendrocytes and may be used as a marker of cell differentiation in the CNS.
Physical form
solution in phosphate buffered saline, containing 0.02% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen 46-48 kDa |
| species reactivity | human, mouse, rat |
| concentration | ~1 mg/mL |
| technique(s) | flow cytometry: 1:200-1:500,immunohistochemistry: 1:100-1:200,western blot: 1:500-1:1,000 |
| UniProt accession no. | P09543 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CNP(1267) mouse ... Cnp(12799) rat ... Cnp(25275) |
