General description
POU domain class 5 transcription factor 1 (POU5F1) is also termed as OCT4. It is a transcription factor. OCT4 codes for three isoforms, such as OCT4A, OCT4B and OCT4B1. These isoforms are produced by alternative splicing. The OCT4 gene consists of 7 exons. This gene is located on human chromosome 6. OCT4A and OCT4B are located in the nucleus and cytoplasm respectively.
Immunogen
synthetic peptide corresponding to an internal sequence of human Oct 3/4. This sequence is 84% identical in mouse and 94% identical in rat.
Application
Anti-Oct 3/4 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence staining.
Biochem/physiol Actions
Oct 3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct 3/4 stimulates loss of pluripotency and dedifferentiation to trophectoderm. POU domain class 5 transcription factor 1 (POU5F1) serves as a master regulator of pluripotency in embryonic stem cells. It is one of the reprogramming factors, that is highly essential for generating induced pluripotent stem cells.
Physical form
solution in phosphate buffered saline, containing 0.02% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| species reactivity | mouse, human, rat |
| concentration | ~1 mg/mL |
| technique(s) | immunohistochemistry: 1:100-1:250,western blot: 1:500-1:1,000 |
| UniProt accession no. | Q01860 |
| application(s) | research pathology |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... POU5F1(5460) mouse ... Pou5f1(18999) rat ... Pou5f1(294562) |
