General description
Tyrosine-protein kinase Lck (UniProt: P06239; also known a EC:2.7.10.2, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, p56-LCK) is encoded by the LCK gene (Gene ID: 3932) in human. Lck is a non-receptor tyrosine kinase that is specifically expressed in lymphoid cells. It plays an essential role in the selection and maturation of developing T-cells in the thymus and also plays a role in function of mature T-cells. Its protein kinase domain is localized to amino acids 245-498. It is reported to constitutively associate with the cytoplasmic region of the CD4 and CD8 receptors. It also plays a role in T-cell antigen receptor (TCR)-linked signaling. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively. This results in recruitment of Lck to the vicinity of the TCR/CD3 complex where Lck can phosphorylate tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR- chains and CD3 subunits that initiates the TCR/CD3 signaling. Once stimulated, the TCR recruits the tyrosine kinase ZAP70 that becomes phosphorylated and activated by Lck. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. Autophosphorylation of Lck on tyrosine 394 increases its enzyme activity, whereas phosphorylation on tyrosine 505 by C-terminal Src kinase (CSK) results in a reduction in its activity. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rossy, J., et al. (2012). Front. Immunol. 3; 167; Bergman, M., et al. (1992). EMBO J. 11(8); 2919-2924).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Tyrosine-protein kinase Lck.
Application
Quality Control Testing
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lck in Ramos cell lysate.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Lck in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Lck peptide with a KD of 1.0 x 10-6 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Lck in one million Jurkat cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lck in Jurkat cell line.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lck in Ramos cell lysate.
Biochem/physiol Actions
Clone 2B20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase Lck. it targets an epitope within 20 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2B20, recombinant monoclonal |
| description | 2B20 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 58.0 kDa,observed mol wt ~55 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_005347 |
| UniProt accession no. | P06239 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... LCK(3932) |
