General description
The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Immunogen
Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Biochem/physiol Actions
The antibody 9E10 can be used to detect the c-Myc tag.
Physical form
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Store at 2-8°C. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | biotin conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | 9E10, monoclonal |
| form | buffered aqueous solution |
| species reactivity | ( Human), (Tagged fusion proteins in all species) |
| concentration | 1 mg/ml |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P01106 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... MYC(4609) |
