General description
TrkA belongs to the Trk proto-oncogene family and is expressed mainly in central and peripheral nervous systems. When TrkA binds to NGF, it autophosphorylates tyrosine residues like-Tyr490, Tyr674, Tyr675, Tyr751, and Tyr785 and subsequently activates many downstream effector proteins. Defects in TrkA lead to congenital insensitivity to pain with anhidrosis. Anti-phospho-TrkA (pTyr490) antibody can be used in immunocytochemistry (1:100, using cultured cells). It is also useful in identifying endogenous levels of phosphorylated Tyr490 in TrkA, TrkB and TrkC. Rabbit anti- phospho-TrkA (pTyr490) antibody is reactive in mouse, human and rat.
Immunogen
synthetic phosphopeptide Tyr490 corresponding to residues surrounding Tyr490 of human TrkA, conjugated to KLH. This sequence is highly conserved in TrkB and TrkC.
Application
Anti-phospho-TrkA (pTyr490) antibody can be used in immunoprecipitation, immunoblotting and immunohistochemistry. It may also be used in western blotting when diluted to ratio of 1:1000 for NGF-treated PC12 cells.
Physical form
Solution in 10 mM sodium HEPES, pH 7.5, and 150 mM sodium chloride containing 100 μg/ml bovine serum albumin and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| mol wt | antigen 140 kDa |
| species reactivity | rat, mouse, human |
| technique(s) | immunocytochemistry: 1:100 using cultured cells,immunohistochemistry (frozen sections): 1:10,immunoprecipitation (IP): 1:250,western blot: 1:1,000 using NGF-treated PC12 cells |
| UniProt accession no. | P04629 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr490) |
| Gene Information | human ... NTRK1(4914) mouse ... Ntrk1(18211) rat ... Ntrk1(59109) |
