General description
Lysine-specific demethylase 1 (LSD1) acts on mono- and dimethylated histones and demethylates them. It has a role in embryogenesis, cell proliferation, spermatogenesis, adipogenesis and chromosomal segregation. Histone H3 peptide methylated at Lysine 4 is a substrate for LSD1. Histone cluster 1 H3 family member E (HIST1H3E) is a variant of histone 3 and is also called as the replicative histone. It is mainly expressed in the S-phase of the cell cycle. The gene encoding this protein is localized on human chromosome 6p22.
Application
Study enzyme kinetics and screen small molecular inhibitors of LSD1 for drug discovery and HTS applications
Biochem/physiol Actions
Histones have an important role in the organization and modification of chromatin. Histone cluster 1 H3 family member E (HIST1H3E) has a role in DNA synthesis during DNA replication and might also be involved in DNA repair. It is deposited at the chromatin at the time of DNA replication-associated chromatin assembly. During herpes simplex virus 1 (HSV-1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin.
Physical form
Supplied as 500 uL of water solution at 200 uM concetration.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube to recover full content of the tube. Aliquot into single use aliquots. Store remaining product in aliquots at -70°C. Note: Avoid freeze/thaw cycles.
| biological source | human |
| assay | ≥90% (HPLC) |
| form | aqueous solution |
| mol wt | 2283 Da |
| packaging | pkg of 500UL |
| concentration | 0.55 mg/mL |
| UniProt accession no. | P68431 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... HIST1H3E(8353) |
