General description
Transcription factor SOX-9 (UniProt P48436; also known SOX-9) is encoded by the SOX9 gene (Gene ID: 6662) in human. SOX family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at about 40 genomic loci and at least 30 SOX genes have been identified. SOX-9 contains one HMG box DNA-binding domain and acts as a key regulator of developmental processes, including male sex determination, normal skeletal development, chondrogenesis, neurogenesis, and neural crest development. SOX-9 is acts downstream of sonic hedgehog signaling and is shown to be essential for the maintenance of multipotent neural stem cells in embryonic and adult central nervous system (CNS). It is not detected in neuroblasts of the adult subependymal zone (SEZ); however, it is expressed by transit amplifying cells and ependymal cells in the same region. SOX-9 is also shown to be essential for the ′switch′ from neurogenesis to gliogenesis in caudal regions of the developing CNS. Up-regulation of SOX-9 is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Scott, CE et al. (2010). Nat. Neurosci. 13(10); 1181-89).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human SOX-9.
Application
Anti-SOX-9, clone 2B10, ZooMAb is a recombinant Rabbit Monoclonal antibody that detects Sox-9 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SOX-9 in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SOX-9 in SW480 cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected SOX-9 in human liver tissue and iPSC-derived liver progenitor organoid sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SOX-9 in E16.5 mouse embryo, mouse nasal cavity, and developing mouse gut tissue sections.
Biochem/physiol Actions
Clone 2B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine SOX-9. It targets an epitope with in 24 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2B10, monoclonal,recombinant monoclonal |
| description | 2B10 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 56.14 kDa,observed mol wt ~65 kDa |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable using 1:100,immunofluorescence: suitable,immunohistochemistry: suitable using 1:100,western blot: suitable using 1:1,000 |
| isotype | IgG |
| UniProt accession no. | P48436 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SOX9(6662) |
