产品介绍
Product Description
Application
Invertase Glycoprotein Standard has been used:
- to generate N-linked glycan library
- as a negative control to study the binding of lectins to high mannose structures
- for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis
用于生产糖果食品和人造蜂蜜。
The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.
Biochem/physiol Actions
转化酶将蔗糖水解成葡萄糖和果糖，产生无色产物，与产生有色产物的酸水解不同。
Other Notes
Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.
技术参数
Specifications
| biological source | Saccharomyces cerevisiae |
| Quality Level | 200 |
| product line | BioReagent |
| form | lyophilized powder |
| mol wt | 60 kDa |
| concentration | ≥0.5 mg/vial protein (E |
| storage temp. | 2-8°C |
