General description
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Immunogen
Whole hairy cell leukemia cells and membrane preparation
Biochem/physiol Actions
The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
Physical form
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Preparation Note
Purified by protein-A affinity chromatography.
Store at 2-8°C. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | unconjugated |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | S-HCL-1, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | 1 mg/ml |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2bκ |
| UniProt accession no. | P20273 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CD22(933) |
