产品介绍
Product Description
Biochem/physiol Actions
NEK6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA family. NEK6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. NEK6 is phosphorylated and activated during M phase of the cell cycle.Inhibition of NEK6 function by either overexpression of an inactive NEK6 mutant or elimination of endogenous NEK6 by siRNA arrests cells in M phase and triggers apoptosis.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
技术参数
Specifications
| recombinant | expressed in baculovirus infected Sf9 cells |
| Quality Segment | 200 |
| product line | PRECISIO® Kinase |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | 114-154 nmol/min·mg |
| mol wt | ~63 kDa |
| UniProt accession no. | Q9HC98 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... NEK6(10783) |
